Safaa Fallatah

Dear Phage Group,

My research Project was about aiming to determine the potential application of bacteriophages for the detection of Listeria spp. on food contact surfaces (FCS). Eight phages were selected for further characterization to determine the most appropriate phage for use in a detection assay. They were characterized for host range, TEM, stability to air dying for 24 h at 25 °C, and restriction endonuclease pattern. L. monocytogenes strain C716 was very resistant to all phages, however; a mutated phage, AG13M, was able to infect L. monocytogenes strain C716. AG20 & AG23 phages were high specificity against their host (Listeria spp). AG20 phage was immobilized on ColorLok paper and used in the to detect L. monocytogenes C519 in broth and FCS. AG20 phage was able to detect as few as 50 CFU/mL of L. monocytogenes in TSB and 40 CFU/cm2 on FCS using a plaque assay to detect progeny phage within 24 h.

I'm so proud to join you and be one of your community. I would like to learn more and dig in depth more about phage matters and all that matters.