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Benjamin Adler (University of California, Berkeley) and colleagues published CRISPRi-ART for phage functional genomics, a new broad-spectrum phage functional genomics platform. It leverages RNA-targeting dCas13d to selectively interfere with protein translation, allowing measurement of phage gene fitness at a transcriptome-wide scale. It works for all kinds of phages, and has already identified more than 90 previously unknown genes important for phage fitness!

SourceCapsid & Tail #300