HIDEN-SEQ: transposon-insertion sequencing that links phage genes to selectable phenotypes (Nature Microbiology)

A Nature Microbiology study from ETH Zürich, University Hospital Basel, the University of Basel and the University of Lausanne presents HIDEN-SEQ (hidden Acr-enabled transposon-insertion sequencing), which systematically links viral genes to selectable phenotypes. Using phage T4 it reproduced the established gene essentiality map, and it is portable to non-model phages, revealing conditionally essential genes in several bacterial hosts and conditions, including previously unknown antidefence factors matched to specific antiviral defences.