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Hans Duyvejonck and colleagues at Ghent University and the Queen Astrid Military Hospital in Belgium have published a paper evaluating the use of qPCR to quantify 5 phages within a cocktail. They compared qPCR to plaque assays, and showed that the qPCR/plaque assay ratio was phage-specific and reproducible. Their results suggest that if a qPCR/plaque assay ratio is determined for a given phage, qPCR can be reliably used to approximate numbers of infectious particles, which could save a lot of time in (especially clinical) phage studies.
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| Source | Capsid & Tail #46 |
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